2024 Aggrecan expression

Aggrecan expression

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August undefined, 2024

WebApr 10, 2024 · The expressions of DYRK1A and its downstream signal epidermal growth factor receptor (EGFR) were detected in the cartilage of adult wild-type mice with … WebExpression of constitutively active (CA)-PI3K significantly induced aggrecan promoter activity. We observed that PI3K maintained Sox9 gene expression and activity: inhibition …

A comprehensive atlas of Aggrecan, Versican, Neurocan …

WebThe Cat-301 monoclonal antibody identifies aggrecan, a chondroitin sulfate proteoglycan in the cat visual cortex and dorsal lateral geniculate nucleus (dLGN). During development, aggrecan expression increases in the dLGN with a … WebJan 4, 2024 · Proper extracellular matrix production is also based on the precise timing of aggrecan expression and its well-regulated turnover. mRNA expression of ADAMTS4, a specific proteolytic enzyme of aggrecane, showed a peak on days 2 and 3 of culturing (Figure 3A). Its protein expression continuously reduced until the end of chondrogenesis … ibps 2021 registration

Cartilage-targeting mRNA-lipid nanoparticles rescue perifocal …

WebSep 29, 2016 · RUNX-2 is a major transcription factor regulating aggrecan loss via the regulation of ADAMTS-4/5 gene expression [29,30] while SOX-9 acts as a master regulator of aggrecanases at both the transcriptional and posttranscriptional levels [31,32,33]. This distinction may explain why some markers appeared to be stronger at the protein level … WebIn addition, TGF‑β1‑induced type II collagen and aggrecan expression were significantly suppressed by ERK1/2 inhibitor PD98059. Similarly, TGF‑β1‑stimulated type II collagen and aggrecan expression were decreased in the presence of a Smad3 phosphorylation inhibitor SIS3. Therefore, the present study demonstrated that the ERK1/2 and ... WebApr 7, 2024 · Optimized conditions of coupled electrical field (60 kHz, 2 mV/mm cyclic stimulation) when applied for 1 h increased aggrecan mRNA expression, and for 6 h—collagen type II; 30 min continuous stimulation reduced expression of MMP-1, MMP-3, MMP-13 and ADAMTS-4, and ADAMTS-5. These effects can be completely blocked by … ibps 2020 vacancy

Aggrecan: Beyond cartilage and into the brain - PubMed

Association of NAT2 genetic polymorphism with the efficacy of ...

WebJun 30, 2024 · To determine whether matrix synthesis and degradation was affected by SNP and/or S-Equol, primary chondrocytes were treated with 0.8 mM SNP and/or 30 μM S-Equol for 24 h, then polysaccharides contents were examined by toluidine blue O staining, type II collagen, and aggrecan gene expression were analyzed by real-time PCR, and MMP-2, … WebAggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. ibps 2021 notification pdfWebFeb 28, 2024 · This suggests that eNOS deficiency may elevate aggrecan expression, which promotes apoptosis in VSMC, thereby contributing to atherosclerosis progression. These results may facilitate the development of novel approaches for improving the diagnosis or treatment of atherosclerosis. ibps-60w

"WebAggrecan is a type of protein known as a proteoglycan, which means it has several sugar molecules attached to it. It is the most abundant proteoglycan in cartilage, a tough, flexible tissue that makes up much of the skeleton during early development. " - Aggrecan expression

Aggrecan expression

Aggrecan - an overview ScienceDirect Topics

WebDownload scientific diagram Western blot analysis of COL2, aggrecan, and MMP-3 in each group. (a) Protein expression levels of COL2, aggrecan, and MMP-3. (b) Semiquantitative analysis of COL2. WebMar 24, 2024 · The expression levels of genes encoding collagen II, aggrecan, Sox9, and Osteocalcin were quantified using RT-PCR on a Real-time PCR thermocycler (ABI Stepone, USA). The sequence of primers (Sinaclon, Iran) that we used in real-time PCR was as Table 2. Table 2 Primer sequences for RT-PCR Full size table

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WebAggrecan (Acan) is the major proteoglycan in articular cartilage and loss of aggrecan is a known characteristic of early OA. In addition to its degradation by cartilage … WebSep 29, 2016 · RUNX-2 is a major transcription factor regulating aggrecan loss via the regulation of ADAMTS-4/5 gene expression [29,30] while SOX-9 acts as a master …

WebApr 13, 2024 · To select the KLF3-AS1 expression-related genes, Pearson’s correlation coefficient was used to analyze the expression correlation based on GSE70362 and GSE124272 (∣r∣ > 0.5, p < 0.05). Six gene (DDHD2, LINC00342, ZBTB20, SUN1, NR3C1, and ZFP90) expressions were positively correlated with KLF3-AS1 expression, and 3 … WebNov 15, 2024 · Previous research revealed that an imbalance in the extracellular matrix (ECM) via abnormal hypoxia-inducible factor-1alpha (HIF-1α) expression in nucleus pulposus (NP) cells was one of the key factors in the pathogenesis of intervertebral disc degeneration (IDD).

WebAggrecan is a type of protein known as a proteoglycan, which means it has several sugar molecules attached to it. It is the most abundant proteoglycan in cartilage, a tough, … WebAggrecan is well-studied in cartilage but its expression and function in the central nervous system has only recently begun to be appreciated. Aggrecan plays an important role in …

WebMar 21, 2024 · In the CNS, aggrecan is expressed in a precise and tightly regulated manner both temporally and spatially. (Review) Increased expression of collagen II, …

WebApr 10, 2024 · The expressions of DYRK1A and its downstream signal epidermal growth factor receptor (EGFR) were detected in the cartilage of adult wild-type mice with destabilized medial meniscus (DMM) and articular cartilage of patients with OA. ibps 2021 vacancy detailsWebExpression of constitutively active (CA)-PI3K significantly induced aggrecan promoter activity. We observed that PI3K maintained Sox9 gene expression and activity: inhibition of PI3K/AKT resulted in decreased Sox9 expression, lowered promoter activity, and mediated a reduction in Sox9 transcriptional activity. ibps 2023 formWebAug 25, 2008 · Digoxigenin-labeled RNA probes for aggrecan and bone sialoprotein (BSP) from our previous studies were used (Fukada et al. 1999; ... Sox5 mRNA was detected in Meckel's anlage at E11.0 before aggrecan mRNA expression. Thus, interactions between Sox9 and Sox5 are also adapted for primary cartilage formation of neural crest in origin. ibps 2022 vacancy detailsWebJun 17, 2024 · Aggrecan is an integral component of the extracellular matrix in cartilaginous tissues, including the growth plate. Heterozygous defects in the aggrecan gene have … ibps 2023 examsWebMay 5, 2007 · These data suggest that aggrecan expression and PN expression may have an important and specific role in activity-dependent plasticity in the rodent somatosensory cortex. Materials and Methods Animals and experimental groups. Experimental animals, CD-1 mice (Charles River Laboratories, Wilmington, MA), were … ibps 2022 cut offWebApr 10, 2024 · Gene expressions of col2a1, aggrecan and sox9 were significantly down-regulated, while expression of MMP13 was significantly up-regulated in defect group compared to control group (Fig. 1 E). Download : Download high-res image (934KB) Download : Download full-size image; Figure 1. Evaluation of perifocal chondrocyte … ibps 2023 registrationWebAggrecan (Acan) is the major proteoglycan in articular cartilage and loss of aggrecan is a known characteristic of early OA. In addition to its degradation by cartilage aggrecanases which play a critical role in the development of OA, decreased aggrecan expression is often evident in OA cartilage. ibps abbreviation