Webincomplete conversion of the peptides after the first coupling. For chloroalkane tagged peptides, 3 equiv. of chloroalkane carboxylate, 3 equiv. of PyBOP and 10 equiv. of DIPEA in DMF were added to piperidine-deprotected peptide and incubated for 1 h. This was repeated if incomplete conversion was indicated via a Kaiser test. WebSep 12, 2024 · We developed a HaloTag-based assay called the chloroalkane penetration assay (CAPA), which is quantitative, high-throughput, and compartment-specific. We demonstrate the ability of CAPA to profile extent of cytosolic penetration with respect to …
Dithiolane quartets: thiol-mediated uptake enables cytosolic delivery ...
WebMay 20, 2024 · More recently, a chloroalkane penetration assay was developed by Foley et al. ( Figure 4) [ 27 ]. This assay uses a cell line that stably expresses a HaloTag–GFP … WebAug 17, 2024 · The chloroalkane penetration assay (CAPA) has been used by our group and others to test the cytosolic penetration of a large variety of peptides, … unbottled test
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WebThe chloroalkane penetration assay (CAPA). (A) Structure of ligand-bound HaloTag (PDB 5UXZ ), with only the linker shown ( Liu et al., 2024 ). Overlay shows a commonly used linker and its relative positioning within the HaloTag active site; blue sphere denotes a molecule-of-interest attached to the chloroalkane and linker. WebJun 6, 2024 · In this study, the recently developed chloroalkane penetration assay was combined with established assays to characterize the cellular uptake and cytosolic delivery of the prototypic cyclotide, kalata B1. We show that kalata B1 enters the cytosol at low efficiency. A structure–activity study of residues in loop 6 showed that some ... WebAug 31, 2024 · We developed a HaloTag-based assay called the chloroalkane penetration assay (CAPA), which is quantitative, high-throughput, and compartment-specific. We … thorntons factory clearance chocolates