Cits857
WebThis was isolated as spontaneous mutant of a lambda Nam7 Nam53 cIts857 prophage. Mycoplasma contamination Not detected Technical information ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. WebNov 30, 1990 · Experiments performed over a temperature range spanning 29-42 degrees C indicate that, under our conditions, temperatures as low as 29 degrees C may be required to fully repress the CI857-controlled transcription from PL, and that the highest protein yields are obtained after induction at 36 degrees C for 6 h. Publication types
Cits857
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WebJan 1, 1994 · The modified cIts857 gene of lambdaphage was obtained by cloning a 990bpDNA (cIindIts857Sam7), modified by deletion of the 3^-noncoding sequence andmutation of two HindIII sites. WebThe cI857 interacts with three operator domains (oL3/oR3, oL2/oR2, and oL1/oR1), repressing transcription. The heterologous gene is localized downstream from the pL or …
WebIn an effort to improve industrial production of 1,3-propanediol (1,3-PD), we engineered a novel polycistronic operon under the control of the temperature-sensitive lambda phage P(L)P(R) promoter regulated by the cIts857 repressor and … http://genome-www.stanford.edu/vectordb/vector_descrip/NOTCOMPL/PJW2.SEQ.html
WebNov 30, 1990 · Experiments performed over a temperature range spanning 29-42 degrees C indicate that, under our conditions, temperatures as low as 29 degrees C may be … WebcIts857基因的克隆,修饰及温敏诱导表达载体. 从噬菌体λDNA (cIindlts857Sam7)克隆出990bP的cIts857基因,经缺失,点突变等修饰改造和DNA序列测定,获得了带有自身启动子 …
WebMar 28, 2007 · Lysogen λD1180 (Dam15 del EcoRI-SacI cIts857 nin5 Sam100) is deficient in gpD, the S lysis gene, and contains a temperature-sensitive mutation in the cI … roof repair pekin ilWebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble … roof repair pickens countyWebY513 h red3 cIts857 H. Nash Y526 h intam29 red3 clts857 H. Nash Y884 h attP24 xisam6 red3 c1ts857 (21) Y885 h xisam6 red3 cIts857 (21) G540 int2l red3 imm434 This work G541 intam2271 red3 imm434 This work G542 intam2111 red3 imm434 This work G548 ga149 int21 red3 imm434 This work G549 ga149 bio2039 red3 imm434 This work G550 ga149 … roof repair petaluma caWebdescribed in Results. This phage carries the cIts857 and nin5 mutations. Xgt-Ec-groE cI+ and Xgt-Ec-groE Qam21 were constructed by recombination with X+ and X Qam21 cIts857. Xgt-Ec-groE cI+ has lost the nin5 deletion as judged by the sensitivity of the virion to inactivation by pyrophosphate; Xgt-Ec-groE Qam21 was not tested for nin5. The E ... roof repair peoria ilWebXX RN [3] RC pJLf201 from phage fd terminator & lambda, cIts857 gene/pR/pL RA McCarthy J.E., Schairer H.U., Sebald W.; RT "Translational initiation frequency of atp genes from Escherichia RT coli: identification of an intercistronic sequence that enhances RT translation"; RL EMBO J. 4:519-526(1985). roof repair peoria azWeb侯云德,男,汉族,1929年7月13日出生于江苏省常州市,中国工程院院士,医学病毒学专家,中国疾病预防控制中心病毒病预防控制所院士实验室主任。1948年至1955年,侯云德本科就读于同济大学医学院;1956年至1957年,侯云德就读于北京俄语学院;1962年至1973年,任中国医学科学院病毒学研究所肝炎 ... roof repair port allenWebThe vector pBV220 contained the λ promoters P R and P L in tandem orientation, the cIts857 gene coding for the thermolabile repressor, and two transcription terminators. roof repair phoenix