http://php.scripts.psu.edu/dept/hafenstein/pdfs/1-s2.0-S0065352719300247-main.pdf WebJun 1, 2024 · More recent variations on the single-particle like approach to helical reconstruction were made in FREALIGN (Alushin et al., 2010, Sachse et al ... asymmetric refinement of a featureless cylinder often does not work. For example, asymmetric refinement of a featureless cylinder never yielded a structure with discernable subunits …
CryoEM reconstruction approaches to resolve asymmetric …
WebJan 1, 2024 · Symmetry relaxation begins with an icosahedral refinement in order to assign orientations, which allows subsequent iterative sampling of the 60 symmetry-related orientations per particle. This approach effectively “relaxes” the higher-order symmetry and allows for the emergence of underlying asymmetric features. WebThe refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. justine mccarthy irish times
Frealign: Getting Started The Grigorieff Lab
WebMore recent variations on the single-particle like approach to helical reconstruction were made in FREALIGN (Alushin et al., 2010, Sachse et al., 2007 ... asymmetric refinement of a featureless cylinder often does not work. For example, asymmetric refinement of a featureless cylinder never yielded a structure with discernable subunits for TMV ... Webnaı¨ve asymmetric refinement may be purposefully biased by introducing an extra density to the reference model at the site of the expected asymme-try, as was done in the case of the RNA polymerase within cytoplasmic polyhedrosis virus (Zhang et al., 2015). 3.3 Symmetry relaxation WebFREALIGN is a program developed by Niko Grigorieff laboratory for high-resolution refinement of 3D reconstruction from cryoEM of single particles. We have modified the original FREALIGN (version v8.06) to run on GPU processors so that the refinement process of single particle 3D reconstruction can be speedup. justine mccarthy md