Periplasmic copper-binding protein
WebJul 19, 2002 · The PcoC protein contains a single copper binding site The PcoC protein is predicted from the DNA sequence of the pcoC gene to be a periplasmic protein [8], and it was purified as described in Materials and methods. WebMar 24, 2016 · The CopC proteins are periplasmic copper binding proteins believed to play a role in bacterial copper homeostasis. Previous studies have focused on CopCs that are part of seven-protein Cop or Pco systems involved in copper resistance. These canonical CopCs contain distinct Cu (I) and Cu (II) binding sites.
Periplasmic copper-binding protein
Did you know?
WebThe CopC protein, reminiscent of the copper chaperones, is a small soluble protein found in the periplasmic space that has been shown to bind one atom of copper. Finally, the CopD protein, which contains several putative transmembrane domains, is thought to be localized to the P. syringae inner membrane. WebApr 1, 2024 · The overall folds of these periplasmic Cu-binding proteins can be categorized into β-barrel (CusF, CopC, PcuC, PcoC, and CopK), α/β-fold (NosL and ScoI), and α-helical bundle (CnrX). Domain of unknown function 305 (DUF305) was first discovered by in …
WebSep 8, 2024 · @article{osti_1901591, title = {Maturation of Rhodobacter capsulatus Multicopper Oxidase CutO Depends on the CopA Copper Efflux Pathway and Requires the cutF Product (Final Report)}, author = {Öztürk, Yavuz and Blaby-Haas, Crysten E. and Daum, Noel and Andrei, Andreea and Rauch, Juna and Daldal, Fevzi and Koch, Hans-Georg}, … WebThe CopC proteins are periplasmic copper binding proteins believed to play a role in bacterial copper homeostasis. Previous studies have focused on CopCs that are part of …
WebCopC is a periplasmic copper-binding protein (17) suggested to work in concert with CopD (15). Copper-inducible expression of these proteins is regulated by the CopRS two-component system. While gene disruption studies have linked these operons to copper resistance (14, 18), expression of the CopC and CopD proteins alone confers copper hy- WebWe have determined the crystal structure of apo-CusF, a periplasmic protein involved in copper and silver resistance in Escherichia coli. The protein forms a five-stranded β-barrel, classified as an OB-fold, which is a unique topology for a copper-binding protein. ... which is a unique topology for a copper-binding protein. NMR chemical shift ...
WebNov 11, 2024 · The copper binding site comprises residues Cys143 and Met147 in the plug domain and His323 and Met325 in the barrel wall. ... Valderrama B. Evolution and diversity of periplasmic proteins involved in copper homeostasis in gamma proteobacteria. BMC Microbiol. 2012;12:249. pmid:23122209 . View Article PubMed/NCBI Google Scholar ...
WebAug 17, 2024 · Genomic and proteomic information associated with selected up‐detected periplasmic proteins in cells producing copper sulfide particles. A) Genomic region of the up‐detected DegP‐like ... the row elizabeth and jamesWebCopper is critically important for methanotrophic bacteria because their primary metabolic enzyme, particulate methane monooxygenase (pMMO), is copper-dependent. In addition to pMMO, many other copper proteins are encoded in the genomes of methanotrophs, including proteins that contain periplasmic copper-A chaperone (PCu A C) domains. Using tractor tom buzz to the rescue dvdWebOct 13, 2014 · The CusCBA complex spans the periplasmic space of Gram-negative bacteria during copper or silver stress and acts to pump out excess metal ions. CusF is believed to … the row elleshopWebAt the host-pathogen interface, macrophages respond to bacterial infection by copper-dependent killing mechanisms, whereas the invading bacteria are thought to counter. … the rowell trading companyWebThe periplasm contains the most numerous and diverse copper-dependent enzymes in bacteria. Accordingly, this compartment is most at risk of copper- induced damage, which is exacerbated under anoxic condi- tions.13To reduce the potential for damage by copper, bacteria have evolved multi-copper oxidases that protect against copper toxicity. the row ellerston sunglassesWebJul 11, 2024 · The periplasmic copper chaperone CusF binds Cu (I) via two important methionine residues [ 18, 24, 25 ]. Also for the periplasmic adaptor CusB, and the inner membrane component CusA, methionine residues play a pivotal part in Cu (I) binding and in the stepwise shuttle mechanism by which the pump extrudes copper from the cell [ 26 – … the rowe londonWebDifferential proteomics suggests that periplasmic proteins, such as a DegP-like protein and a heavy metal-binding protein, could be involved in this process. The unexpected periplasmic formation of copper sulfide nanoparticles in BW-1 reveals previously unknown possibilities for intracellular biomineralization. the rowen atl