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Thp-1 macrophage-like cells

WebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours and rested in RPMI 1640 with 10% FBS for another 24 hours; for KFs and NFs, 3 × 10 4 cells pretreated with 500 nM AS1517499 (or DMSO control) were added to the upper 24-well ... WebIt is concluded that the cGNSCD204 are promising to trace the differentiation of THP-1 cells into macrophages in their live condition. As one imaging method to evaluate monocyte …

Construction method of mononuclear macrophage model based on HIV-1 …

WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … WebApr 13, 2024 · HIGHLIGHTS. who: Maria Rynikova and colleagues from the Department of Animal Physiology, Faculty of Science, Pavol Jozef Safarik University in have published … fan club address https://acquisition-labs.com

Lysine-Specific Histone Demethylase 1A Regulates Macrophage ...

WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as … WebMay 20, 2024 · Immunomodulatory proteins from human milk may enhance the protection and development of the infant’s gut. This study compared the immunomodulatory effects … WebMar 28, 2024 · Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh WW, Halu A, Yamada I, Yoshida H, Hara T, Wei M, Inoue N, Fukuda D, Mojcher A, Mattson PC, Barabasi AL, … core keeper caveling id

Apolipoprotein A-I promotes cholesterol release and …

Category:Frontiers A THP-1 Cell Line-Based Exploration of Immune …

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Thp-1 macrophage-like cells

A Novel Triple-Cell Two-Dimensional Model to Study Immune …

WebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which … WebDec 1, 2024 · Macrophages are the preferential cell types to study various aspects of mycobacterial infection. Commonly used infection models for in-vitro studies are primary …

Thp-1 macrophage-like cells

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WebMay 2, 2024 · In the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated … WebHere, macrophage‐like THP‐1 cells, exposed to ten different CNM for 48 h in low‐cytotoxic concentration of 10 µg mL−1, are characterized by secretion of ... epithelial type-II BEAS …

WebTHP-1 cells (human monocytic cell line) were cultured in 6-well cell culture plates (1×10 6 cells per well) and maintained in complete RPMI 1640 medium at 37°C. For differentiation … WebSep 4, 2024 · In the co-culture system, THP-1 cells were induced to the M2-like state by SW480 cells. The level of miR-148a negatively correlated with the levels of M2-related cytokines. ... and the macrophage cells that migrated to the lower surface were fixed with formalin and stained with 0.1% crystal violet.

WebJan 1, 2024 · The take up rate by an immortalized macrophage-like cell line of a number of mesoporous silica nanoparticles with differing diameter and pore size ... {Macrophage … WebJun 26, 2024 · Experiments were performed on human THP-1, a human monocyte-like cell line derived from a patient with leukaemia, obtained from ATCC bank (ATCC# ... Repnik U et al (2024) Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines. Altex 35:211–222. PubMed Google Scholar Latz E, Xiao TS , Stutz A (2013 ...

WebThe THP-1 cells were purchased from Sigma Aldrich and maintained in a humidified incubator at 37oC with 5% CO 2 in RPMI 1640 (Gibco) ... (Gibco). Cells were seeded at 5 x …

WebIn general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. … core keeper caveling bossWebTo examine the impact of macrophage-secreted factors on cell behavior, 12Z cells on all three substrates were treated with conditioned media from differentiated THP-1 (an … fanclub anoukWebFigure 1 IL-17 induces M2-like phenotype in THP-1-derived macrophages. Notes: (A) THP-1-derived macrophages were treated with IL-17 at 0, 10, 50 and 100 ng/mL for 48 hours. The … core keeper caveling scholarWebLoss of ME-1 in THP-1 cells was also associated with reduced efferocytosis. ME-1 is a target gene of NRF2 (nuclear factor erythroid 2–related factor 2), and the authors have … fanclub aufnäherWebSep 27, 2024 · THP-1 is a human monocyte leukaemia cell line, which differentiates into macrophage-like cells when treated with phorbol esters. When differentiated, THP-1 … fanclub andrea bergWebMigration assays showed that conditioned media from T98G cGAS cells increased the migration of THP-1 cells as compared to that from T98G Empty cells (Fig 5A). However, … fan club agnetha faltskogWebAug 1, 2024 · It has been shown that THP-1 derived macrophage-like cells (THP-1 Mφ) resemble primary Mφ in some functional properties and differentiation markers (Chanput … core keeper caveling bread